The practice of physical activity is an important component in maintaining health and well-being. This study aimed to verify if there is an association between physical activity practice and substance use among university students. An analytical, descriptive, cross-sectional study involved 1,169 students (50.4% male; 63.3% aged 20-29 years; 92.8% single), from different courses at a public university located in the inland state of Minas Gerais, in southeastern Brazil. Sociodemographic Questionnaire, Alcohol, Smoking and Substance Involvement Screening Test, and Baecke Habitual Physical Activity Questionnaire were applied. The results show a prevalence of binge drinking (47.2%). Marital status, age group, alcohol consumption, tobacco, marijuana, and cocaine have a negative impact on levels of physical activity practice. In conclusion, university students engage in little physical activity, which may contribute to an increase in unhealthy behaviors, including alcohol and/or other substance consumption.

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